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| Section | Weight | Objectives |
|---|---|---|
| Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Quality control and quality assurance - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance |
| Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Semen and amniotic fluid testing |
| Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing |
| Clinical Chemistry | 20-25% | - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring |
| Immunology and Serology | 5-10% | - Immunologic testing methodologies - Immune system principles and components - Infectious disease serology - Autoimmune and immunodeficiency disorders |
| Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Transfusion reactions and adverse events - Blood group systems and antigens - Compatibility testing - Antibody detection and identification |
| Microbiology | 15-20% | - Mycology, parasitology, and virology - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Identification of pathogenic microorganisms |
1. Hepatitis B surface antigen, or HBs, is a protein that is present on the surface of the virus. The protein will be present via laboratory testing in the blood with acute and chronic HBV infections Immunology What sero-marker is the first marker to become positive in Hepatitis B and is associated with the infective stage?
A) HBc total
B) Hbe antigen
C) Hbs antibody
D) HBc antibody
E) HBs antigen
2. Which EBV markers would be MOST likely positive for an individual who had infectious mononucleosis 9 years ago?
A) None of the markers would be positive after 9 years
B) Anti-VCA (IgM) and heterophile
C) All would still be positive
D) Anti-VCA (IgG) and anti-EBNA
3. As a general rule, CSF glucose is about two thirds of the serum glucose measured in a normal adult.
Chemistry
Which of the following would be considered a normal cerebrospinal fluid glucose if the serum glucose is 70 mg/dl?
A) 60 mg/dl
B) 70 mg/dl
C) 45 mg/dl
D) 100 mg/dl
E) 30 mg/dl
4. The membranes of ion selective electrodes will determine which analyte is measured by the electrode.
Chemistry
The selectivity of an ion-selective electrode is determined by the:
A) magnitude of the potential across the membrane internal reference electrode
B) type of glass used in electrode
C) properties of the membrane used
D) solution used to fill the electrode
5. Allergen-specific IgE, synthesized in response to allergens, becomes fixed to receptors on cellular membranes, especially those of basophils. If these receptor-bound IgE molecules are aggregated on re-exposure to specific allergen, both mast cells and basophils produce mediators that result in the allergic response. IgE-antigen interaction at the cell surface causes degranulation of cells and release of substances including: histamine, SRS-A, platelet activator, a kallikrein, and an eosinophil chemotactic factor. Basophils are the principal cells that bind IgE antibody while their number of receptor sites is proportional to serum IgE levels. Eosinophils are drawn to the site by the basophil chemotaxis mechanism, but are not the main cell which binds the IgE antibody.
Immunology
The mediator cells that bind MOST to IgE antibodies are:
A) Eosinophils
B) Macrophages
C) Basophils
D) Polymorphonuclear neutrophils
Solutions:
| Question # 1 Answer: E | Question # 2 Answer: D | Question # 3 Answer: E | Question # 4 Answer: C | Question # 5 Answer: C |
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