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American Society of Microbiology ABMM Sample Questions:

1. Staphylococcus epidermidis, a common cause of healthcare-associated infections, forms biofilms on medical devices. The production of polysaccharide intercellular adhesin (PIA) is a key factor in biofilm maturation.
The expression of PIA is primarily regulated by:

A) A two-component regulatory system involving AgrA and AgrC.
B) The icaADBC operon, whose expression is influenced by environmental cues.
C) Horizontal gene transfer of the ica operon.
D) The stringent response pathway activated during nutrient limitation.

2. During a primary infection with Epstein-Barr virus (EBV), the virus establishes latency in B lymphocytes.
The transition from lytic to latent infection is tightly regulated by viral gene expression. Which of the following viral proteins is crucial for the establishment and maintenance of EBV latency and is a key target in EBV-associated lymphomas?

A) Epstein-Barr nuclear antigen 1 (EBNA1)
B) Glycoprotein gp350
C) Viral capsid antigen (VCA)
D) Early antigen (EA)

3. A patient undergoing chemotherapy for leukemia develops a severe pneumonia. Bronchoalveolar lavage fluid is examined, revealing numerous cysts containing crescent-shaped structures upon silver staining. The patient's CD4+ T-cell count is significantly low. The MOST likely diagnosis is infection with:

A) Pneumocystis jirovecii
B) Coccidioides immitis
C) Blastomyces dermatitidis
D) Histoplasma capsulatum

4. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

A) The number of true positive results divided by the total number of positive test results.
B) The number of true negative results divided by the total number of true negative and false positive results.
C) The number of true negative results divided by the total number of negative test results.
D) The number of true positive results divided by the total number of true positive and false negative results.

5. Microscopic examination of a potassium hydroxide (KOH) preparation of skin scrapings from a patient with tinea versicolor typically reveals:

A) Cigar-shaped yeast cells
B) Septate hyphae and arthroconidia
C) Broad, aseptate hyphae with sporangiophores
D) Short, unbranched hyphae and globose yeast cells in clusters ("spaghetti and meatballs")

Solutions: